epitope tagging meaning in English
表位附加,表位追加
表位附加，表位追加[把附加的抗原表位融合到目的蛋白上
Examples
- In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers , the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells , and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e . coli m15 . the following main results and conclusions can be obtained from the present study : 1 . the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method , and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag . the recombinant plasmid was identified by polymerase chain reaction , restriction endonuclease maping and sequencing , this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
为了进一步研究axud1在人类肿瘤发生中的作用及axud1基因的表达状况与tgf -介导的信号通路的关系，本实验研究分为三个部分： ( 1 ) axud1基因cdna全长编码区的克隆和ha表位标记的axud1基因表达载体的构建； ( 2 )探讨肝癌细胞hepg2和肺腺癌spc - a1细胞中tgf - 1诱导的axud1基因表达的时间、剂量效应以及诱导表达的可能机理，并研究axud1的过表达对细胞周期和细胞凋亡相关蛋白表达的影响； ( 3 ) axud1原核表达载体的构建及其在大肠杆菌中的表达。本实验的主要结果和结论如下： 1利用一步法rt - pcr成功地从人类外周血淋巴细胞中克隆出axud1基因编码区cdna ，并将其构建入真核表达载体中，编码的ha - axud1融合蛋白带有流感病毒凝血素ha的表位标记肽段。